from agent-almanac17
Design and optimize an HPLC method (RP, NP, IC, HILIC) including column selection, mobile phase, gradient, and detection for robust analytical separations.
Guides analysts through designing and validating HPLC methods for separating and quantifying non-volatile analytes. Covers chromatographic mode selection (RP, NP, IC, HILIC), column choice, mobile phase composition and pH control, gradient design, detector configuration (UV/DAD/MS), method verification, and validation checks.
Use when developing new analytical methods for QA/QC, transferring methods between instruments, optimizing selectivity, or converting to UHPLC. Applicable in pharmaceutical, chemical, and food testing labs.
Best used by lab-focused agents and code assistants that can format method SOPs and generate parameter tables for instruments (Lab automation, Copilot-style agents).
This skill has not been reviewed by our automated audit pipeline yet.
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